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Updated: Jan 11, 2026

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Sox8 is essential for vertebrate gastrulation.

Sofia Moreira1,2, Artemis G Korovesi3, Elias H Barriga4,5

  • 1Mechanisms of Morphogenesis Lab, Cluster of Excellence Physics of Life (PoL), TU Dresden, Dresden, Germany.

EMBO Reports
|November 10, 2025
PubMed
Summary

Sox8 is a key regulator of Xenopus laevis gastrulation, controlling cell movements and body axis formation. Its depletion causes developmental defects by modulating Wnt signaling through kremen2 activation.

Keywords:
Xenopus laevisGastrulationKremen2Sox8Ventrolateral Mesoderm

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Gastrulation is crucial for forming germ layers and body axes.
  • Precise cell movements and fate specification orchestrate gastrulation.
  • Transcription factors play pivotal roles in regulating developmental processes.

Purpose of the Study:

  • Identify novel regulators of Xenopus laevis gastrulation.
  • Investigate the role of SOXE transcription factors in Xenopus gastrulation.
  • Elucidate the molecular mechanisms underlying Sox8-mediated regulation of gastrulation.

Main Methods:

  • CRISPR-DiCas7-11 for Sox8 depletion in Xenopus laevis.
  • Transcriptomic analysis to identify downstream targets of Sox8.
  • Chromatin immunoprecipitation to confirm Sox8 binding to target gene promoters.
  • Whole mount in situ hybridization to analyze gene expression patterns.

Main Results:

  • Sox8 is expressed in the ventrolateral mesoderm and is essential for blastopore closure and AP axis elongation.
  • Sox8 directly activates the transcription of kremen2, a Wnt signaling inhibitor.
  • Sox8 and Kremen2 knockdown disrupt Wnt signaling, leading to abnormal mesodermal patterning and reduced BMP signaling.

Conclusions:

  • Sox8 is a critical regulator of Xenopus gastrulation, controlling key developmental events.
  • The Sox8/Kremen2 regulatory axis modulates Wnt signaling to ensure proper mesodermal patterning.
  • This study provides new insights into the molecular control of vertebrate gastrulation.