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Selective Assembly between Cu2+ and β-Nicotinamide Mononucleotide (NMN).

Jialun He1, Jiazhuo Li2, Ying Wang1

  • 1Analytical & Testing Center, Sichuan University, Chengdu 610064, China.

Inorganic Chemistry
|November 11, 2025
PubMed
Summary
This summary is machine-generated.

Nicotinamide mononucleotide (NMN) selectively binds with copper ions (Cu2+) to form a precipitate. This unique coordination chemistry highlights the importance of the N+ site in NMN for selective metal ion assembly.

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Area of Science:

  • Coordination Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Nucleotides possess N/O coordination sites, facilitating versatile assembly with diverse metal ions.
  • The interaction between nucleotides and metal ions is crucial in various biological and chemical processes.

Purpose of the Study:

  • To investigate the selective assembly of β-nicotinamide mononucleotide (NMN) and its derivatives with metal ions.
  • To elucidate the structural and chemical basis for selective metal ion coordination in nucleotides.

Main Methods:

  • Selective precipitation experiments with NMN and various metal ions, including Cu2+.
  • Comparative analysis of NMN, NMNH, NAD+, and NADH in their assembly with Cu2+.
  • Isothermal titration calorimetry (ITC) to quantify binding affinities.

Main Results:

  • NMN selectively assembled with Cu2+ to form a distinct blue-green precipitate.
  • The reduced form, NMNH, and NADH did not exhibit similar selective precipitation with Cu2+.
  • The N+ site in the pyridine moiety of NMN was identified as essential for selective Cu2+ assembly.
  • ITC data indicated favorable binding of Cu2+ with NMN compared to other divalent metal ions.

Conclusions:

  • The N+ site in NMN is critical for its selective coordination with Cu2+.
  • A balance between electrostatic repulsion and binding affinity likely drives the selective assembly process.
  • This study reveals a unique aspect of nucleotide coordination chemistry with implications for metal ion sensing and complex formation.