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Improved efficacy of an influenza DNA vaccine through high-density microarray patch delivery
Chloe G Entriken1, Kimberley L Bruce1, Bridget M Coyne1
1School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, 4072, Australia.
The Journal of General Virology
|November 14, 2025
Summary
High-density microarray patches (HD-MAPs) improve DNA vaccine delivery for pandemic preparedness. This novel approach enhances immunogenicity and protection against influenza, overcoming limitations of traditional intramuscular injection.
Area of Science:
- Vaccinology
- Biotechnology
- Infectious Disease Research
Background:
- Pandemic preparedness necessitates rapid, stable, and deployable vaccine platforms.
- DNA vaccines offer advantages but face immunogenicity challenges with intramuscular (IM) delivery.
Purpose of the Study:
- To evaluate high-density microarray patch (HD-MAP) delivery of an influenza DNA vaccine.
- To compare HD-MAP delivery with conventional IM injection for immunogenicity and efficacy.
Main Methods:
- HD-MAP delivery of a DNA vaccine encoding the H1N1pdm09 haemagglutinin (HA) antigen.
- In vivo imaging to assess antigen expression.
- Measurement of HA-specific IgG responses.
- Viral challenge studies in mice.
Main Results:
- HD-MAP delivery showed earlier and higher antigen expression compared to IM injection.
- HD-MAP vaccination induced strong HA-specific IgG responses, unlike IM delivery.
- All HD-MAP-vaccinated mice were protected from weight loss after H1N1 challenge, versus 50% in the IM group.
Conclusions:
- HD-MAPs effectively overcome DNA vaccine delivery limitations.
- This technology enhances DNA vaccine immunogenicity and protective efficacy.
- HD-MAPs represent a promising platform for future pandemic response strategies.
