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Related Experiment Video

Updated: Jan 11, 2026

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

Bidirectional quantitative scattering microscopy.

Kohki Horie¹, Keiichiro Toda², Takuma Nakamura²

¹Department of Physics, The University of Tokyo, Tokyo, Japan.

Nature Communications

|November 14, 2025

Summary

This summary is machine-generated.

Bidirectional quantitative scattering microscopy (BiQSM) combines forward and backward scattering for label-free imaging. This new technique simultaneously visualizes nanoscale and microscale cellular structures, overcoming limitations of prior methods.

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Last Updated: Jan 11, 2026

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

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Published on: April 7, 2014

Area of Science:

Biomedical imaging
Cellular dynamics
Quantitative phase microscopy

Background:

Quantitative phase microscopy (QPM) and interferometric scattering (iSCAT) microscopy are label-free techniques with complementary strengths and weaknesses.
QPM excels at microscale imaging but struggles with nanoscale dynamics.
iSCAT is sensitive to nanoscale dynamics but limited in microscale imaging.

Purpose of the Study:

To develop a novel imaging technique that integrates the capabilities of QPM and iSCAT.
To enable simultaneous, high-resolution imaging of both nanoscale and microscale cellular components.
To advance label-free monitoring of dynamic biological processes.

Main Methods:

Introduction of bidirectional quantitative scattering microscopy (BiQSM).
Integration of forward scattering (FS) and backward scattering (BS) detection.
Utilized off-axis digital holography with bidirectional illumination and spatial-frequency multiplexing.

Main Results:

BiQSM achieved spatiotemporal consistency and a 14x wider dynamic range than QPM.
Enabled simultaneous imaging of nanoscale and microscale cellular structures.
Demonstrated visualization of intracellular structures, small particles, and cellular vital states.

Conclusions:

BiQSM successfully bridges the gap between QPM and iSCAT, offering enhanced quantitative cellular imaging.
The technique allows for comprehensive spatiotemporal analysis of cellular components at multiple scales.
BiQSM holds significant potential for studying dynamic biological processes and cellular health monitoring.