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A rapid pupillometry protocol for clinical use: effect of age and test-retest repeatability
Jason C Park1, J Jason McAnany2,3
1Department of Ophthalmology and Visual Sciences, University of Illinois Chicago, 1855 W. Taylor St., MC/648, Chicago, IL, 60612, USA.
Documenta Ophthalmologica. Advances in Ophthalmology
|November 15, 2025
Summary
A new, rapid pupillometry protocol using a portable device was developed. This method accurately measures pupillary light reflex (PLR) responses, showing good repeatability and potential for clinical use.
Area of Science:
- Ophthalmology
- Neuroscience
- Medical Devices
Background:
- Traditional pupillometry often requires specialized, non-portable equipment and lengthy protocols.
- A need exists for rapid, clinically applicable pupillometry methods.
Purpose of the Study:
- To develop and evaluate a swift, portable pupillometry protocol.
- To assess the clinical utility of a handheld instrument for pupillometry.
Main Methods:
- Thirty-seven healthy adults underwent pupillary light reflex (PLR) testing using chromatic flashes.
- Experiments evaluated dark adaptation time, age effects, and test-retest repeatability.
- Key metrics quantified included baseline pupil size (BL), maximum pupil constriction (MPC), and post-illumination pupillary response (PIPR).
Main Results:
- A 3-minute dark adaptation period was found sufficient for maximal PLRs.
- Pupillary responses (BL, MPC, PIPR) showed good test-retest repeatability (approx. 1 mm for BL, 10% for MPC/PIPR).
- Age did not significantly impact PLR metrics, with PIPR being independent of age.
Conclusions:
- The developed protocol is effective for measuring MPC and PIPR across various ages and baseline pupil sizes.
- The portable device and short protocol (approx. 5 min) offer promising clinical utility.
- This rapid pupillometry approach enhances diagnostic capabilities in clinical settings.
