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Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
Published on: December 19, 2020
Calcium-loaded lipopeptides for enhanced bioavailability and reduction of calcium deficiency
Zahra Rokhsatzadeh1, Maryam Fanaei1, Giti Emtiazi2
1Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Abstract:
This study investigates the development of lipopeptide-based calcium complexes to enhance calcium bioavailability. Two distinct lipopeptide components were extracted from Bacillus mojavensis HF: a solvent-extracted lipopeptide (LP), and an extracellular lipopeptide composite (ELC), obtained through salting-out. Initial tests revealed that ELC was more negatively charged and exhibited superior efficacy in dissolving CaCO3, leading to the selection of ELC for complex preparation. Two types of ELC-based complexes were prepared using CaCO3 and CaCl2, and characterization techniques confirmed the incorporation of calcium salts into the ELC matrix. Energy Dispersive X-ray Spectroscopy (EDX) analysis showed the presence of 25 % calcium in the ELC/CaCO₃ complex and 15 % in the ELC/CaCl₂ complex. Thermogravimetric Analysis (TGA) revealed that CaCO3 exhibited superior thermal stability, with significant degradation occurring above 300 °C. The solubility and particle size analysis revealed that the complexes exhibit significant solubility under simulated intestinal conditions, even in the presence of oxalic acid. This suggests a strong potential for enhanced calcium bioavailability. This study is the first report on the application of a bacterial lipopeptide composite to enhance the bioavailability of calcium, which could represent a step toward developing new calcium supplement formulations.
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