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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Jan 10, 2026

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

3D-Printed CRISPR-based detection system powered by a reusable handwarmer.

Daniel Schreiber^1,2, Rui Yang^1,2, Xin Guan^1,2

¹Department of Biomedical Engineering, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA.

Biomedical Microdevices

|November 22, 2025

Summary

This summary is machine-generated.

This study presents a low-cost, 3D-printed, electricity-free system for CRISPR-based nucleic acid detection. The reusable system uses a handwarmer for heat and achieves high sensitivity for HPV-16 DNA detection.

Keywords:

3D printing CRISPR-Cas12a HPV-16 Microfluidic chip Sodium acetate

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Published on: February 3, 2021

Area of Science:

Biotechnology
Molecular Diagnostics
Point-of-Care Testing

Background:

Nucleic acid diagnostics are crucial for cancer and infectious disease management.
Existing methods often require expensive equipment and electricity, limiting accessibility.

Purpose of the Study:

To develop a 3D-printed, electricity-free system for CRISPR-based nucleic acid detection.
To create a low-cost, reusable platform for point-of-care molecular testing.

Main Methods:

Fabricated a 3D-printed microfluidic chip with integrated fluid transport and reaction chamber.
Utilized a reusable heating platform powered by a sodium acetate handwarmer and a docosane wax heatsink for temperature regulation.
Integrated a lateral flow strip for visual readout of CRISPR-based detection.

Main Results:

Demonstrated analytical performance by detecting HPV-16 DNA with a sensitivity as low as 1 femtomolar.
Validated clinical feasibility using cervical samples, with results comparable to standard PCR assays.
Achieved successful nucleic acid detection without external electrical power.

Conclusions:

The developed system is a low-cost, reusable, and electricity-free solution for molecular diagnostics.
This technology offers a practical approach for point-of-care testing, especially in resource-limited settings.
The system demonstrates potential for widespread application in infectious disease and cancer screening.