Related Experiment Video
Updated: Jan 10, 2026
09:11
Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
Published on: January 27, 2023
2.7K
Flow Cytometric Determination of Micronucleus Frequency.
Azeddine Elhajouji1, Pascal Stadelmann2
1Genome Safety, Preclinical Safety, Biomedical Research, Novartis Pharma AG, Basel, Switzerland. Azeddine.elhajouji@novartis.com.
Methods in Molecular Biology (Clifton, N.J.)
|November 22, 2025
Summary
The micronucleus (MN) test is a versatile biomarker for chromosomal damage, widely used in genotoxicity screening. This chapter details MN test protocols and flow cytometry analysis for accurate assessment.
Area of Science:
- Toxicology
- Genetics
- Biomarkers
Background:
- The micronucleus (MN) test is a widely adopted genotoxicity screening tool.
- MNs are biomarkers of chromosomal damage or mitotic abnormalities.
- The assay is applicable to various cell types, in vitro and in vivo.
Purpose of the Study:
- To provide an overview of protocol designs for the MN test.
- To describe experimental steps for successful MN test performance.
- To detail accurate MN analysis using flow cytometry.
Main Methods:
- Micronucleus (MN) test protocol design.
- In vitro and in vivo MN testing guidelines (OECD 487, OECD 474).
- Flow cytometry for MN analysis.
Main Results:
- The MN test is a versatile tool for understanding genotoxin effects.
- Standardized guidelines (OECD) enhance its regulatory and research utility.
- Flow cytometry offers accurate MN analysis.
Conclusions:
- The MN test is crucial for genotoxicity assessment in research and regulatory settings.
- This chapter provides practical guidance on MN test execution and analysis.
- Flow cytometry is a key technique for accurate MN quantification.
