Related Experiment Video
Updated: Jan 10, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
Published on: July 5, 2016
Computational optical sectioning in Fresnel incoherent correlation holography
Vijayakumar Anand1,2, Joseph Rosen3,4
1Institute of Physics, University of Tartu, W. Ostwaldi 1, 50411, Tartu, Estonia. vijayakumar.anand@ut.ee.
Abstract:
Fresnel incoherent correlation holography (FINCH) is a widely used incoherent digital holography technique. FINCH has a higher lateral resolution but a lower axial resolution compared to those of direct imaging methods with the same numerical aperture. The low axial resolution problem of FINCH was addressed by developing sectioning FINCH methods implemented by pixel-by-pixel electronic scanning of a phase pinhole to achieve sectioning capability in FINCH, mimicking a conventional confocal microscope. The above approach requires not only an additional spatial light modulator for electronic scanning but also time-consuming data acquisition and processing. In this study, for the first time, to the best of our knowledge, we developed a fully computational sectioning method for FINCH. This computational optical sectioning FINCH (COS-FINCH) exploits the axial intensity and phase characteristics of FINCH holograms and first and second order derivatives of axial intensity distributions to identify the object planes, extract information, and achieve sectioning. Extensive simulation studies and results of preliminary experimental studies are presented.
Related Concept Videos
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Super-resolution Fluorescence Microscopy
Total Internal Reflection Fluorescence Microscopy
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

