Related Experiment Video
Updated: Jan 10, 2026
11:22
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
31.0K
Identification of the Novel Allele, HLA-DQB1*03:577, by Next-Generation Sequencing
Antonio Giuseppe Bianculli1, Paola Giustiniani1, Tiziana Galluccio1
1Laboratory of Immunogenetics and Transplant, Complex Operative Unit of Oncohematology, Hematopoietic Transplantation and Cellular Therapies, IRCCS Bambino Gesù Pediatric Hospital, Rome, Italy.
HLA
|November 24, 2025
Summary
A novel Human Leukocyte Antigen (HLA) variant, HLA-DQB1*03:577, has been identified. This new allele differs from the common HLA-DQB1*03:01:01:01 by a single nucleotide substitution.
Area of Science:
- Immunogenetics
- Molecular biology
- Human leukocyte antigen (HLA) research
Background:
- The Human Leukocyte Antigen (HLA) system plays a critical role in immune response.
- Polymorphisms in HLA genes, particularly HLA-DQB1, are associated with various autoimmune diseases.
- Accurate HLA typing is essential for transplantation and disease association studies.
Purpose of the Study:
- To characterize a newly identified HLA-DQB1 allele.
- To document the specific genetic variation distinguishing the novel allele from a known common allele.
Main Methods:
- Nucleotide sequencing of the HLA-DQB1 gene.
- Comparison of the novel sequence with existing HLA reference sequences.
Main Results:
- A novel HLA-DQB1 allele, designated HLA-DQB1*03:577, was identified.
- This new allele differs from HLA-DQB1*03:01:01:01 by a single nucleotide substitution (G>A) at position 5565 in the exon 4 region.
Conclusions:
- The identification of HLA-DQB1*03:577 expands the known HLA-DQB1 allele repertoire.
- This specific nucleotide substitution may have implications for immune response and disease susceptibility.
Related Concept Videos
03:00Next-generation Sequencing
97.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.6K
01:29Modern Molecular Taxonomy
554
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
554