Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.9K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.9K
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

5.2K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
5.2K
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

3.9K
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
3.9K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.7K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.7K
Centrosome Duplication02:25

Centrosome Duplication

4.8K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.8K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.3K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond Accuracy: Safety-Centered guidelines for the evaluation of LLM-based therapy recommendation systems for chronic multimorbidity patients.

Journal of biomedical informatics·2026
Same author

A lipid cue drives the subcellular localization of a self-inserting bacterial transmembrane protein.

bioRxiv : the preprint server for biology·2026
Same author

Experience-Dependent Gain Modulation Drives Thermosensory Responses in Behavior.

bioRxiv : the preprint server for biology·2026
Same author

A 3D-printed osteochondral scaffold with a dual biomimetic design of spatially organized lotus-radial microchannels and bioinspired nano-mineral precursors for efficient osteochondral regeneration.

Biofabrication·2026
Same author

Efficient spatio-angular reconstruction enables high-fidelity mapping of six-dimensional structures and dynamics with polarized fluorescence microscopy.

Research square·2026
Same author

Super-Resolution Structured Illumination Microscopy to Study Endocytosis and Cellular Nanoparticle Uptake.

ACS nano·2026

Related Experiment Video

Updated: Jan 10, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.6K

The CENP-A chaperone complex spatially organizes centromeres.

Hindol Gupta1, Julian Haase1, Yicong Wu2

  • 1Laboratory of Biochemistry & Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH; Bethesda, 20892, USA.

Biorxiv : the Preprint Server for Biology
|November 24, 2025
PubMed
Summary

Before mitosis, the CENP-A chaperone complex builds shell-like structures at centromeres, creating chromatin-poor cavities. This architecture is crucial for cell division and genome stability.

More Related Videos

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A

Published on: June 10, 2020

6.0K
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.3K

Related Experiment Videos

Last Updated: Jan 10, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.6K
Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A

Published on: June 10, 2020

6.0K
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.3K

Area of Science:

  • Cell Biology
  • Epigenetics
  • Molecular Biology

Background:

  • Centromeres are essential for chromosome segregation during cell division.
  • CENP-A nucleosomes and the constitutive centromere-associated network (CCAN) form inner kinetochore structures.
  • The organization of these components before mitosis remains largely unknown.

Purpose of the Study:

  • To investigate the interphase structure of centromeres.
  • To understand the role of the CENP-A chaperone complex in centromere organization.
  • To elucidate the mechanism of centromere preparation for mitosis.

Main Methods:

  • Immunofluorescence microscopy
  • Super-resolution imaging
  • Biochemical assays to study protein interactions

Main Results:

  • Interphase CENP-A and CCAN form ~200-300 nm shell structures with a central chromatin-poor cavity.
  • The interphase-specific CENP-A chaperone complex occupies this cavity.
  • Chaperone presence, not CENP-A incorporation, drives shell formation and chromatin clearing.
  • The CCAN scaffold CENP-C is radially organized and essential for structure formation.

Conclusions:

  • The CENP-A chaperone machinery plays a critical structural role in interphase centromere organization.
  • This machinery establishes centromere architecture prior to mitosis.
  • This configuration is vital for faithful kinetochore assembly and genome stability.