On Temporal Robustness & Brain-State Stability of Functional Connectivity in Mouse Primary Visual Area V1 compared to
Mario Alexios Savaglio1,2, Christina Brozi1,2, Stelios M Smirnakis3
1Department of Computer Science, University of Crete, Greece.
Biorxiv : the Preprint Server for Biology
|November 26, 2025
Summary
Functional connectivity in the higher-order visual area anterolateral area (AL) is more stable over time and across brain states than in the primary visual cortex (V1). AL
Area of Science:
- Neuroscience
- Systems Neuroscience
- Visual Cortex Function
Background:
- Understanding neural coordination in the visual cortex is key to information processing.
- Higher-order visual areas (e.g., AL) have distinct roles and sustained representations compared to V1.
- The stability of functional connectivity architecture in higher-order areas across time and states is poorly understood.
Purpose of the Study:
- To compare the stability of functional connectivity architecture between V1 and AL across different brain states.
- To investigate how visual stimulation and resting states affect functional connectivity in these areas.
- To determine the influence of arousal on functional connectivity in V1 versus AL.
Main Methods:
- In vivo mesoscopic two-photon calcium imaging in mice.
- Simultaneous recording of neuronal activity in V1 and AL.
- Analysis of functional connectivity using the spike time-tiling coefficient (STTC) under visual stimulation and resting states.
Main Results:
- Functional connectivity in AL is consistently more robust over time than in V1.
- AL's functional connectivity structure shows less change between stimulus and resting states compared to V1.
- AL activity and connectivity are less influenced by pupil size (arousal) during rest than V1.
Conclusions:
- Functional connectivity in higher-order area AL is more stable and less state-dependent than in V1.
- Spontaneous activity in AL better reflects stimulus-evoked network architecture.
- Arousal has a weaker modulatory effect on AL compared to V1, suggesting distinct network dynamics.
Keywords:
functional connectivityhigher visual areas (HVA)network neuroscienceprimary visual cortex (area V1)visual cortexMore Related Videos
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