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Updated: Jan 10, 2026

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
Published on: January 7, 2019
Structure and gene cluster of the capsular polysaccharide produced by Acinetobacter baumannii K111
Andrei V Filatov1, Anna M Shpirt1, Andrei V Perepelov1
1N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
None:
Structure of the capsular polysaccharide (CPS) from A. baumannii MAR21-2688 strain (classified as KL111 type) was established using 1H and 13C NMR spectroscopy, including two-dimensional homonuclear 1Н,1Н COSY, TOCSY, ROESY and heteronuclear 1Н,13C HSQC and HMBC experiments and chemical methods including component analyses and Smith degradation. The following structure of the branched hexasaccharide repeating K-unit was established: Functions of genes in the K locus of A. baumannii K111 were assigned by a comparison with sequences in the available databases and found to be in agreement with the CPS structure.
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