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Related Experiment Video

Updated: Jan 10, 2026

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

Spheroid Sheets: A Scalable Platform for Producing Tissue Membrane Constructs.

Quang Bach Le¹, Hariharan Ezhilarasu¹, Weng Wan Chan¹

¹Biomanufacturing Technology (BMT)/Bioengineering & Automation (BE&A), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), Singapore.

|November 27, 2025

Summary

This summary is machine-generated.

This study introduces a novel, scalable method for assembling cell spheroids into thin tissue sheets using a frame and mesh system. This scaffold-free approach enables controlled thickness and efficient nutrient diffusion for tissue engineering applications.

Keywords:

Bioengineering Biomanufacturing Cartilage Cell spheroids Cell therapy Mesenchymal stem cells Tissue engineering Tissue membrane

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Last Updated: Jan 10, 2026

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Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

Published on: March 31, 2022

Area of Science:

Tissue Engineering
Biomaterials Science
Regenerative Medicine

Background:

Bottom-up tissue engineering using cell spheroids mimics native tissue environments.
Existing spheroid assembly methods lack control over geometry, scalability, and throughput.
Developing scalable and controllable methods is crucial for advancing tissue engineering.

Purpose of the Study:

To present a robust and scalable method for assembling cell spheroids into thin, planar tissue sheets.
To demonstrate the utility of this method for creating cartilage tissue constructs.
To provide a practical platform for generating membrane-like tissue for research and therapy.

Main Methods:

Cell spheroids are assembled between two flexible meshes held by a frame.
The frame and mesh system guides spheroid fusion, controls thickness, and ensures nutrient exchange.
Human mesenchymal stem cells were used to create chondrogenic cartilage tissue.

Main Results:

A thin, planar spheroid sheet tissue construct was successfully produced.
The method allows for controlled tissue thickness and uniform spheroid fusion.
The resulting scaffold-free constructs are easily handled and can be transplanted.

Conclusions:

The described frame-and-mesh method offers a scalable and robust approach to spheroid-based tissue engineering.
This technique facilitates the creation of thin, membrane-like tissue constructs for diverse applications.
The method overcomes limitations of previous spheroid assembly techniques, enhancing tissue engineering capabilities.