Related Experiment Video
Updated: Jan 10, 2026
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
Published on: November 13, 2017
Protocol for transcriptomic and epigenomic analysis of JAK inhibitor sensitivity in IFN-γ-primed human macrophages
Geunho Kwon1, Junho Noh1, Jaehyun Lee1
1Department of Biological Sciences and Biotechnology, Chungbuk National University, Cheongju 28644, Republic of Korea.
Abstract:
Here, we present a protocol for integrating transcriptomic and epigenomic data to analyze Janus kinase (JAK) inhibitor sensitivity in interferon (IFN)-γ-primed human macrophages using assay for transposase-accessible chromatin using sequencing (ATAC-seq) and RNA sequencing (RNA-seq). We describe steps for ATAC-seq data generation, differential accessibility analysis, and transcription factor motif enrichment. We then detail procedures for integration with RNA-seq data. This systematic approach identifies epigenomic regulatory mechanisms underlying differential JAK inhibitor sensitivity. For complete details on the use and execution of this protocol, please refer to Kwon et al.1.
