Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jan 10, 2026

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
11:25

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

10.8K

Visualizing the translation landscape in human cells at high resolution.

Wei Zheng1, Yuekang Zhang1, Jimin Wang1

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.

Nature Communications
|November 28, 2025
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CryoGO enables high-resolution structural profiling of endogenous cellular macromolecules.

bioRxiv : the preprint server for biology·2026
Same author

Cytoplasmic lattices store developmentally poised degradative and cytoskeletal complexes in mammalian eggs.

Nature structural & molecular biology·2026
Same author

Something old, something new? Herpesvirus genome packaging examined in light of lessons from the tailed bacteriophages.

Journal of virology·2026
Same author

Structural basis of multimodal adsorption and infection initiation by <i>Vibrio</i> phage Peru-2.

bioRxiv : the preprint server for biology·2026
Same author

Functional traits mediate the effects of soil property on the growth performance of Ardisia gigantifolia cuttings.

BMC plant biology·2026
Same author

Lattice-Induced 2D Ti<sub>3</sub>C<sub>2</sub>T<sub><i>x</i></sub> MXene and COF Supramolecular Nanohybrids for Electrocatalytic Water Splitting.

Langmuir : the ACS journal of surfaces and colloids·2026

Researchers mapped human protein synthesis in cells using advanced cryo-electron microscopy (cryo-EM). This revealed detailed ribosome structures and functional states, offering new insights into cellular processes.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Cell Biology

Background:

  • Understanding macromolecular structures in situ is crucial for advancing biology and human health.
  • Previous studies often relied on in vitro methods, potentially missing native cellular context.

Purpose of the Study:

  • To map the in situ structure of human protein synthesis machinery.
  • To investigate functional states and interactions of the human 80S ribosome within its native cellular environment.

Main Methods:

  • Utilized automated cryo-focused ion beam (FIB) milling combined with in situ single-particle cryo-electron microscopy (cryo-EM).
  • Achieved high-resolution (2.2 Å) consensus structure of the human 80S ribosome.
  • Analyzed structures from cells treated with specific inhibitors (homoharringtonine, cycloheximide).

More Related Videos

Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

Published on: August 22, 2017

10.4K
Bead Loading Proteins and Nucleic Acids into Adherent Human Cells
07:28

Bead Loading Proteins and Nucleic Acids into Adherent Human Cells

Published on: June 1, 2021

5.3K

Related Experiment Videos

Last Updated: Jan 10, 2026

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
11:25

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

10.8K
Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

Published on: August 22, 2017

10.4K
Bead Loading Proteins and Nucleic Acids into Adherent Human Cells
07:28

Bead Loading Proteins and Nucleic Acids into Adherent Human Cells

Published on: June 1, 2021

5.3K

Main Results:

  • Resolved 23 functional states of the human 80S ribosome, mostly at resolutions better than 3 Å.
  • Observed structural variations, distinct ion/polyamine binding environments, and associated proteins (EDF1, NACβ) not typically found in purified ribosomes.
  • Detected peptide-related densities, visualized ribosome-ribosome interactions in polysomes, and identified spermidine interaction with cycloheximide.

Conclusions:

  • High-resolution in situ cryo-EM provides unprecedented insights into native cellular processes.
  • Revealed a complex translational landscape and the role of polyamines in ribosome function.
  • Highlights the importance of studying macromolecules in their native cellular context for biological understanding.