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Updated: Jan 9, 2026
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Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant
Published on: September 7, 2016
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Strontium implantation improving cell proliferation and adhesion on titanium surfaces through CaSR/AKT signaling
Qingyong Chen1, Bingkun Cheng2, Qingling Gao1
1Department of Stomatology, Handan Central Hospital.
Dental Materials Journal
|November 30, 2025
Summary
Strontium-doped titanium (Sr-Ti) enhances fibroblast adhesion and proliferation, improving soft tissue integration for implants. This study reveals Sr-Ti activates the calcium-sensing receptor (CaSR) pathway, crucial for better implant biocompatibility and success.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Surface Chemistry
Background:
- Surface modifications improve implant biocompatibility and soft tissue integration.
- Titanium (Ti) is a common implant material, but its surface properties can be enhanced.
- Strontium (Sr) incorporation is explored for its potential biological benefits.
Purpose of the Study:
- To evaluate the effects of Sr-doped Ti materials on fibroblast behavior.
- To investigate the underlying molecular mechanisms of Sr-induced fibroblast adhesion.
- To assess Sr-functionalized Ti as a strategy for improved implant integration.
Main Methods:
- Magnetron sputtering to create Sr-doped Ti plates.
- Assessment of fibroblast (L929) proliferation, morphology, and adhesion strength.
- Surface characterization using SEM, EDX, and AFM.
- Molecular analysis via RNA sequencing and Western blot, including CaSR inhibition.
Main Results:
- Sr-doped Ti significantly enhanced L929 fibroblast proliferation and adhesion.
- Surface analysis confirmed Sr incorporation and altered surface properties.
- Sr promoted fibroblast adhesion by activating the CaSR/PKC/AKT signaling pathway.
- Inhibition of CaSR reversed the pro-adhesion effects of Sr.
Conclusions:
- Sr-functionalized Ti implants promote fibroblast adhesion and early soft tissue integration.
- The CaSR/PKC/AKT pathway mediates the positive effects of Sr on fibroblast adhesion.
- Sr-doped Ti offers a promising approach to enhance implant success and prevent peri-implant diseases.
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