π-d Conjugated Coordination Nanoparticles Engineered by CuNi-MOF: A Rationally Designed NIR-II Photothermal Platform...

Area of Science:

• Materials Science
• Nanotechnology
• Biomedical Engineering

Background:

• Developing efficient photothermal materials for NIR-II bioassays is challenging.
• Strong NIR-II absorption and high photothermal conversion efficiency (PCE) are crucial for these applications.

Purpose of the Study:

• To design and synthesize π-d conjugated coordination nanoparticles for NIR-II photothermal detection of alkaline phosphatase (ALP).
• To establish a sensitive and selective platform for biomarker detection using NIR-II photothermal signals.

Main Methods:

• Synthesized Ni(DMPD^ISQ)₂ nanoparticles via a reaction involving CuNi-BTC and 4,5-dimethyl-1,2-phenylenediamine (DMPD).
• Utilized pyrophosphate (ppi) to modulate nanoparticle formation for ALP detection.
• Employed 1064 nm laser excitation for photothermal signal generation and detection.

Main Results:

• Achieved strong NIR-II absorption and a high PCE of 42.7% with the synthesized Ni(DMPD^ISQ)₂ nanoparticles.
• Demonstrated quantifiable NIR-II photothermal signal generation modulated by ALP's substrate, ppi.
• Obtained a low detection limit (0.44 mU mL^-1), excellent selectivity, and high accuracy (94.7-104.2% recoveries) in human samples.

Conclusions:

• The developed π-d conjugated coordination nanoparticles serve as high-performance NIR-II photothermal transducers.
• The study presents a versatile and sensitive platform for advanced NIR-II photothermal bioassays.
• This work opens new avenues for sensitive biomarker detection in biological samples.