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Interspecies rice versus Arabidopsis thaliana protein-protein interactome profiling by touch-down overlapping PCR
Jie Huang1, Yu Cheng1, Jing Ruan1,2
1State Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.
Journal of Integrative Plant Biology
|December 4, 2025
Abstract
None:
Touch-down overlapping PCR coupled with HiFi long-read sequencing, a high-throughput method for large-scale profiling of protein-protein interactions based on stitch-PCR identified 7,726 high-confidence interactions between rice and Arabidopsis proteins by integrating a library-vs-library yeast two-hybrid strategy with optimized PCR and long-read sequencing.
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