Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bone-inspired (GNEC/HAPAAm) hydrogel with fatigue-resistance for use in underwater robots and highly piezoresistive sensors.

Microsystems & nanoengineering·2023

Same author

Promising Natural Medicines for the Treatment of High-Altitude Illness.

High altitude medicine & biology·2023

Same author

3D Porous Cu-Composites for Stable Li-Metal Battery Anodes.

ACS nano·2023

Same author

The mediating role of exhaled breath condensate metabolites in the effect of particulate matter on pulmonary function in schoolchildren: A crossover intervention study.

The Science of the total environment·2023

Same author

Ultra-Long Cycle of Prussian Blue Analogs Achieved by Equilibrium Electrolyte for Aqueous Sodium-Ion Batteries.

Small (Weinheim an der Bergstrasse, Germany)·2023

Same author

Identifying immune cell infiltration and diagnostic biomarkers in heart failure and osteoarthritis by bioinformatics analysis.

Medicine·2023

Search research articles

Related Experiment Video

Updated: Jan 9, 2026

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Published on: September 22, 2013

StainExpert: A Unified Multi-Expert Diffusion Framework for Multi-Target Pathological Stain Translation.

Zeyu Liu, Yufang He, Tianyi Zhang

IEEE Transactions on Medical Imaging

|December 8, 2025

Summary

StainExpert is a new AI framework that generates multiple virtual pathology stains from a single image. This computational pathology tool streamlines diagnostics, saves tissue, and improves efficiency.

More Related Videos

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Published on: November 14, 2025

Related Experiment Videos

Last Updated: Jan 9, 2026

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Published on: September 22, 2013

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Published on: November 14, 2025

Area of Science:

Computational pathology
Digital pathology
Artificial intelligence in medicine

Background:

Histopathological analysis is crucial for disease diagnosis, using various staining methods like hematoxylin and eosin (H&E), special stains, immunohistochemistry (IHC), and multiplex immune-fluorescence (mpIF).
Current sequential staining methods are time-consuming, labor-intensive, consume significant tissue, and can compromise sample integrity due to serial sectioning.

Purpose of the Study:

To introduce StainExpert, a unified multimodal diffusion framework for translating a single source pathological stain image into multiple target stain images.
To develop a novel multi-expert system that enables efficient, single-source to multi-target stain translation, overcoming limitations of single-pair models.

Main Methods:

Developed StainExpert, a multimodal diffusion framework with a multi-expert system for collaborative learning of staining principles.
Employed multi-expert and multi-objective optimization for efficient source-to-multi-target translation.
Integrated textual guidance with visual features within a multimodal diffusion architecture.

Main Results:

StainExpert successfully generated high-quality virtual stains across H&E, special stains, IHC, and mpIF modalities on three datasets.
The generated virtual stains preserved critical pathological features essential for accurate diagnosis.
The framework demonstrated robust cross-domain generalization capabilities.

Conclusions:

StainExpert offers a transformative platform for efficient multi-target pathological stain translation.
The tool advances computational pathology towards streamlined, tissue-conserving, and resource-efficient diagnostic workflows.
This approach enhances diagnostic accuracy and efficiency in histopathology.