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Quantification of oil/water emulsion stability kinetics using image analysis: ImageScan method.

Luis Condezo-Hoyos1, Sebastián Lama-Quispe2, Zahid Apaza-Medrano2

  • 1Innovative Technology, Food and Health Research Group, Facultad de Industrias Alimentarias, Universidad Nacional Agraria La Molina, Av. La Molina s/n, Lima, Peru; Instituto de Investigación de Bioquímica y Biología Molecular, Universidad Nacional Agraria La Molina, Av. La Molina s/n, Lima, Peru.

Food Chemistry
|December 9, 2025
PubMed
Summary

A new image-based method, ImageScan, quantifies oil-in-water emulsion stability. This cost-effective technique accurately predicts emulsion half-life, offering a reliable tool for stability assessment.

Keywords:
Accelerated stability testEmulsion kinetic stabilityGreen microanalytical assayImage stability indexLow-cost assay

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Area of Science:

  • Physical Chemistry
  • Colloid and Surface Science

Background:

  • Emulsion stability is crucial in various industries, requiring accurate and efficient assessment methods.
  • Traditional methods for evaluating emulsion stability can be time-consuming and costly.

Purpose of the Study:

  • To develop and validate a kinetic, cost-effective, image-based method (ImageScan) for quantifying oil-in-water (O/W) emulsion stability.
  • To establish ImageScan as a reliable tool for determining emulsion half-life time (HLT).

Main Methods:

  • Oil-in-water emulsions were prepared using commercial emulsifiers.
  • Images were captured using a desktop scanner over time.
  • The Image Stability Index (ISI) was calculated from the relative gravitational volume (rGV), and half-life time (HLTi) was determined using the ImageScanEM program.

Main Results:

  • The ImageScan method demonstrated a strong correlation (r = 0.9019, p < 0.05) with traditional kinetic and turbidimetric methods (HLTt).
  • Bland-Altman analysis showed a 95% agreement limit between HLTi and HLTt values.
  • The method effectively quantifies and identifies instability layers in emulsions.

Conclusions:

  • The ImageScan method provides a validated, kinetic, and cost-effective approach for assessing O/W emulsion stability.
  • ImageScan facilitates accurate quantification and identification of emulsion instability.
  • This technique offers a valuable alternative for routine emulsion stability testing.