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Beam shaping on a fluorescent on-chip imaging system.

Çağlar Arpali1, Serap Altay Arpali2

  • 1Department of Mechatronics Engineering, Çankaya University, Yukarıyurtçu Mahallesi Eskişehir Yolu 29. Km, Mimar Sinan Caddesi No: 4, 06790, Etimesgut/Ankara, Türkiye.

Methods and Applications in Fluorescence
|December 10, 2025
PubMed
Summary

Gaussian beam illumination improves signal-to-noise ratio (SNR) in fluorescent on-chip imaging systems, especially in scattering media. This technique enhances particle detection compared to plane-wave illumination, optimizing image quality for dense biological samples.

Keywords:
fluorescent imaginglaser beam shapingon-chip imaging systemssignal-to-noise ratio

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Area of Science:

  • Biophotonics
  • Microscopy
  • Optical Engineering

Background:

  • Fluorescent on-chip imaging places samples directly on sensors (e.g., charge-coupled device (CCD)) for wide field of view and rapid scanning.
  • Dense and scattering environments (e.g., whole blood, tissue) challenge particle detection, reducing signal-to-noise ratio (SNR).
  • Image quality is affected by medium light transmission and excitation methods.

Purpose of the Study:

  • To quantitatively analyze the impact of beam shaping techniques on SNR in fluorescent on-chip imaging.
  • To compare Gaussian beam and plane-wave illumination for particle detection in microchannels.

Main Methods:

  • Developed a novel phase modulation schema to generate Gaussian beam and plane-wave illumination.
  • Utilized a developed imaging platform for experimental comparison.
  • Quantitatively assessed SNR based on image quality.

Main Results:

  • Gaussian beam illumination yielded higher SNR images compared to plane waves.
  • Demonstrated enhanced detection of fluorescent particles in a microchannel using Gaussian beams.
  • Confirmed Gaussian beam's superior energy confinement ability.

Conclusions:

  • Gaussian beam shaping is advantageous for improving image quality in fluorescent on-chip imaging.
  • This technique is particularly beneficial for imaging in scattering-like media.
  • Optimized beam shaping enhances the performance of on-chip imaging systems for biological samples.