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Published on: March 7, 2018
A defect-engineered bimetallic MOF nanozyme with enhanced activity for colorimetric detection of acid phosphatase
Hongbin Wu1, Jinbo Cao1, Xianglin Liao1
1Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, GDMPA Key Laboratory for Process Control and Quality Evaluation of Chiral Pharmaceuticals, School of Chemistry, South China Normal University, Guangzhou, 510006, PR China.
Abstract:
Nanozyme-based colorimetric biosensors offer significant benefits, including operational simplicity, visual readout, cost-effectiveness, and robust stability. However, the catalytic activity and efficiency are highly dependent on their composition and structure, necessitating precise structural optimization. Herein, a novel metal-organic framework nanozyme featuring a defect-like structure (His-MOF) was developed, which exhibited significantly enhanced peroxidase-like activity, as evidenced by the intensified color reaction in the 3,3',5,5'-tetramethylbenzidine (TMB)-H2O2 system. Leveraging the inhibitory effect of ascorbic acid (AA) on the TMB-H2O2 reaction, we established a sensing mechanism wherein L-ascorbic acid-2-phosphate trisodium salt (AAP) is hydrolyzed by acid phosphatase (ACP) to generate AA. Based on this principle, a highly sensitive and low-cost colorimetric biosensor was developed for ACP detection, demonstrating a linear range from 0.2 to 7.0 mU/mL with a limit of detection (LOD) of 0.075 mU/mL. The developed biosensor was successfully employed to determine ACP activity in human serum samples, demonstrating its promising potential for clinical diagnostics. This nanozyme-based biosensing platform facilitates the development of ultrasensitive diagnostics for a broad spectrum of diseases.
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