Comprehensive metabolite profiling of 10-methoxycamptothecin in rats by ultraperformance liquid chromatography
Chen-Xue Song1, Zi-Ming Wang1, Mei-Jia Yan1
1National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.
Abstract:
10-Methoxycamptothecin (MCPT), a natural camptothecin derivative, shows notable anticancer activity. Using UPLC-QTOF-MS, this study characterized MCPT metabolite profiles in rats, detecting 14 metabolites in urine, 5 in bile, 7 in feces, and 2 in plasma. The findings indicated that phase I metabolites of MCPT were primarily formed via demethylation, demethoxylation, and hydroxylation, while phase II metabolites mainly included glucuronide and sulfate of MCPT and its phase I metabolites. MCPT, hydroxycamptothecin, and the subsequent glucuronide conjugates were the predominant forms of MCPT in rats. This study provided a comprehensive overview of the metabolite profile of MCPT in rats.
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