Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.9K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bridging quantum noise and classical electrodynamics with stochastic methods.

Nature communications·2026
Same author

Dual-Ion Based Magneto-ionic Effects in Nanoporous Pd<sub>75</sub>Co<sub>25</sub> Alloy.

ACS materials Au·2026
Same author

Optofluidic Force Induction for Online Monitoring of Particle Size Distributions in Emulsion Polymerization Reactions.

ACS polymers Au·2026
Same author

Low-Energy Single-Electron Detector with Submicron Resolution.

ACS photonics·2026
Same author

Universal photonic processor for spatial mode decomposition.

Nature communications·2025
Same author

Optofluidic Force Induction: A Workbench for Nanoparticle Characterization and Material Analytics.

Nano letters·2025
Same journal

Recent Progress in on-Demand Transfer-Enabled Integration of Wavelength-Scale Light Sources.

Nanophotonics (Berlin, Germany)·2026
Same journal

Tunable skyrmion bag textures in surface phonon polariton lattices.

Nanophotonics (Berlin, Germany)·2026
Same journal

All-Optical Diffractive Operators for Rapid, Computer-Free Morphological Transformations.

Nanophotonics (Berlin, Germany)·2026
Same journal

Tunable Skyrmion, Meron, and Skyrmion Bag Textures in Surface Phonon Polariton Lattices.

Nanophotonics (Berlin, Germany)·2026
Same journal

Deep-Subwavelength Slot-Enhanced Broadband Dynamic Camouflage Metasurface Across the S, C, X, and Ku Bands.

Nanophotonics (Berlin, Germany)·2026
Same journal

Machine Learning-Driven Cooling Window Design Beyond Hyperbolic Metamaterials.

Nanophotonics (Berlin, Germany)·2026
See all related articles

Related Experiment Video

Updated: Jan 8, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.0K

Optimizing the localization precision in coherent scattering microscopy using structured light.

Ulrich Hohenester1, Felix Hitzelhammer1, Georg Krainer2,3

  • 1Institute of Physics, University of Graz, Universitätsplatz 5, 8010 Graz, Austria.

Nanophotonics (Berlin, Germany)
|December 17, 2025
PubMed
Summary
This summary is machine-generated.

This study optimizes excitation fields for precise localization of small scatterers using quantum Fisher information. Optimized fields enhance localization accuracy in microscopy by maximizing field strength and detected photons.

Keywords:
optical coherence microscopy; Fisher information; structured light

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.3K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.0K

Related Experiment Videos

Last Updated: Jan 8, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.0K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.3K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.0K

Area of Science:

  • Optics and Photonics
  • Quantum Metrology

Background:

  • Coherent scattering microscopy requires precise localization of small particles.
  • Optimizing excitation fields is crucial for enhancing localization precision.

Purpose of the Study:

  • To optimize focused excitation fields for enhanced localization precision of small scatterers.
  • To maintain fixed total incoming excitation field intensity during optimization.

Main Methods:

  • Utilizing quantum Fisher information for optimization of excitation fields.
  • Analyzing optimal field polarization (linear, circular, radial) based on numerical aperture (NA).
  • Evaluating performance in interferometric scattering microscopy (iscat).

Main Results:

  • Optimal fields exhibit linear/circular polarization for low NA and radial polarization for high NA.
  • High localization precision correlates with high field strengths and increased detected photons.
  • Optimized fields demonstrate improved performance in iscat.

Conclusions:

  • Quantum Fisher information provides an effective framework for optimizing microscopy excitation fields.
  • Field polarization plays a critical role in achieving high localization precision.
  • The optimized fields show promise for advanced scattering microscopy techniques like iscat.