Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.7K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.7K
MicroRNAs01:22

MicroRNAs

23.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction notice to "Targeting oncogenic functions of miR-301a in head and neck squamous cell carcinoma by PI3K/PTEN and MEK/ERK pathways" [Biomedicine & Pharmacotherapy 161 (2023) 114512].

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Longitudinal Monitoring of Metabolic Gradients in Microreactor Culture Platforms by Raman Spectroscopy.

Biosensors·2026
Same author

Post-processing dynamics of acrylamide in food: a comprehensive review on the influence of storage conditions.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2026
Same author

Risk Assessment in Food Safety.

Foods (Basel, Switzerland)·2026
Same author

Fibroblast Activation Protein Alpha (FAP) Expression Is Associated with Disease Recurrence and Poor Response to Tyrosine Kinase Inhibitors in Advanced Clear Cell Renal Cell Carcinoma.

International journal of molecular sciences·2025
Same author

Inorganic Contaminants in Rapadura from Latin America.

Foods (Basel, Switzerland)·2025
Same journal

Comprehensive Human Health Risk Assessment of Metal(loid)s in Selected Fish Species and Clams From Nun River, Bayelsa State, Nigeria.

Journal of applied toxicology : JAT·2026
Same journal

Long-Term Hepatorenal Toxicity Assessment of the Vasopressin Receptor Antagonist Conivaptan in Lewis Rats: Implications for Therapeutic Safety.

Journal of applied toxicology : JAT·2026
Same journal

BPA and Male Reproductive Health: Mechanistic Insights, Toxicological Implications, and the Protective Role of Antioxidants.

Journal of applied toxicology : JAT·2026
Same journal

Dosimetry of Inhaled Aerosols: From Exposure to Target Tissue Dose in Risk Assessment and Drug Delivery.

Journal of applied toxicology : JAT·2026
Same journal

Emerging Links Between PFAS Exposure and Autoimmune Thyroid Disease: A Narrative Review of Epidemiologic Evidence, Mechanistic Insights, and Research Gaps.

Journal of applied toxicology : JAT·2026
Same journal

RETRACTION: Predictive Performance of the Vitrigel-Eye Irritancy Test Method Using 118 Chemicals.

Journal of applied toxicology : JAT·2026
See all related articles

Related Experiment Video

Updated: Jan 8, 2026

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.9K

MicroRNA Expression During Long-Term Treatment With 3-MCPD and Glycidol In Vitro.

Renan G Tivanello1, María Armesto2, Adriana P Arisseto1

  • 1Faculty of Food Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil.

Journal of Applied Toxicology : JAT
|December 17, 2025
PubMed
Summary
This summary is machine-generated.

Food contaminants 3-monochloropropane diol (3-MCPD) and glycidol can cause kidney cancer. This study found they alter microRNA (miRNA) expression in renal cells, suggesting a new mechanism for their toxicity.

Keywords:
cell culturechronic exposurecontaminantsin vitrorenal cancer

More Related Videos

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
11:53

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Published on: June 1, 2018

7.0K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.3K

Related Experiment Videos

Last Updated: Jan 8, 2026

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.9K
An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
11:53

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Published on: June 1, 2018

7.0K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.3K

Area of Science:

  • Toxicology
  • Molecular Biology
  • Oncology

Background:

  • 3-MCPD and glycidol esters are food processing contaminants.
  • Their free forms are toxic, linked to renal cancer.
  • The role of microRNAs (miRNAs) in their toxicity is unclear.

Purpose of the Study:

  • Investigate miRNA expression changes in renal cells exposed to 3-MCPD and glycidol.
  • Explore the link between these contaminants and renal carcinogenesis.
  • Understand the molecular mechanisms of toxicity.

Main Methods:

  • Exposure of human renal cell lines (786-0, HK-2, HEK-293) to 3-MCPD and glycidol.
  • Assessment of cell viability and toxicity (IC50 determination).
  • RT-PCR analysis of miRNA expression profiles.

Main Results:

  • Glycidol exhibited higher toxicity than 3-MCPD in renal cell lines.
  • Significant upregulation of miRNAs associated with renal carcinogenesis was observed.
  • Downregulation of tumor suppressor miRNAs was detected.

Conclusions:

  • Glycidol and 3-MCPD induce distinct miRNA expression changes in renal cells.
  • These miRNA alterations may contribute to renal carcinogenesis.
  • Findings offer insights into the molecular mechanisms of contaminant-induced kidney cancer.