High-speed holotomography of live cells and tissues using multi-pattern sparse axial scanning

Holotomography has enabled label-free 3D imaging of biological samples but is often limited by slow image acquisition speed. To address this limitation, we propose a fast-scanning scheme, in which multiple patterns are used sparsely along the axial direction. When compared to existing methods, our method quadruples the imaging speed while maintaining image quality. Experimental results on cells and tissues demonstrate the practicality of our method for fast and precise refractive index imaging.