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Noninvasive Dynamic Cell Viability Monitoring Based on Diffraction Height Fingerprint Spectra
Zhaoyuan Zhang1, Tongge Li1, Haodong Liu1
1School of Electrical and Information Engineering, Jiangsu University, Zhenjiang 212003, China.
Analytical Chemistry
|December 19, 2025
Summary
This study introduces a novel label-free method using cell diffraction fingerprint imaging for real-time, noninvasive monitoring of cell viability. The technique effectively tracks apoptosis stages and drug cytotoxicity, advancing cell analysis and drug screening.
Area of Science:
- Biomedical Engineering
- Optical Imaging
- Cell Biology
Background:
- High-throughput cell viability monitoring is crucial for disease and drug efficacy studies.
- Existing methods often lack noninvasive, real-time tracking capabilities throughout the cell cycle.
Purpose of the Study:
- To develop a label-free, dynamic monitoring method for cell viability.
- To enable noninvasive, real-time tracking of cellular changes.
- To provide a tool for mechanism-of-action studies and drug screening.
Main Methods:
- Utilized cell diffraction fingerprint imaging based on cell height characteristic spectra.
- Analyzed diffraction patterns to visualize and quantify cell viability evolution.
- Employed Gray-Level Co-occurrence Matrix (GLCM) texture parameters for cell clustering based on viability.
Main Results:
- Successfully resolved the four distinct stages of apoptosis.
- Demonstrated effective clustering of cells according to viability state using GLCM parameters.
- Validated pharmacological applicability by assessing drug combination cytotoxicity against cancer cells.
Conclusions:
- Presents a paradigm for continuous, noninvasive, single-cell-level viability monitoring.
- Offers a robust tool for mechanism-of-action studies, cellular assessment, and high-throughput drug screening.
- Provides a foundation for label-free optical imaging in cell pathology and biomedical engineering.
