Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.9K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.0K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.0K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.7K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.7K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.9K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

740
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
740
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unlocking complex optical vortices with flat optics.

Reports on progress in physics. Physical Society (Great Britain)·2026
Same author

Green channel-informed collaborative filtering for high-fidelity laser speckle contrast imaging in dual-display laparoscopy.

Biomedical optics express·2026
Same author

Functional analysis of lncRNAs regulating the TOR signaling pathway and reproduction in the white-backed planthopper, Sogatella furcifera.

Pest management science·2026
Same author

Single-shot dual-modality quantitative phase and polarization microscopy.

Optics express·2026
Same author

High-capacity directional information processor using all-optical multilayered neural networks.

Science advances·2025
Same author

Comprehensive Transcriptomic Analysis of Brain Tissues From the Infarcted Area of MCAO Rats Revealed That Acupuncture Attenuates Brain Injury via the Complement System.

Brain and behavior·2025
Same journal

Spatially and Chemically Specific Optical Control of Cells via Supervised and Automated Target Selection.

ACS photonics·2026
Same journal

Coupling Single Molecules to DNA-Based Optical Antennas with Position and Orientation Control.

ACS photonics·2026
Same journal

Interface States in Space-Time Photonic Crystals: Topological Origin, Propagation, and Amplification.

ACS photonics·2026
Same journal

Mid-Infrared Sensing and Ultrafast Photoresponse in Silicon-Based Plasmonic Detectors.

ACS photonics·2026
Same journal

Light-Driven Topological Relaxation and Dynamic Scaling in Photoresponsive Polymer Films.

ACS photonics·2026
Same journal

Electro-optic Modulation in Polycrystalline Barium Titanate Metasurfaces Enhanced by Poling.

ACS photonics·2026
See all related articles

Related Experiment Video

Updated: Jan 8, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.6K

Vector Vortex Beam-Enabled Edge Microscopy with Dynamic Orientation Selectivity.

Hammad Ahmed1, Muhammad Afnan Ansari1, Lynn Paterson2

  • 1Institute of Photonics and Quantum Sciences, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS, U.K.

ACS Photonics
|December 22, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a new edge enhancement imaging system using vector vortex beams for orientation-selective detail highlighting. The technology offers dynamic polarization control for improved visualization in microscopy and diagnostics.

Keywords:
edge imagingoptical metasurfacesoptical vortex beamvector vortex beam

More Related Videos

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
08:13

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

Published on: April 8, 2019

17.9K
Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
09:36

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

4.4K

Related Experiment Videos

Last Updated: Jan 8, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.6K
A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
08:13

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

Published on: April 8, 2019

17.9K
Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
09:36

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

4.4K

Area of Science:

  • Optics and Photonics
  • Image Processing
  • Biomedical Imaging

Background:

  • Edge enhancement is crucial for visualizing fine details in low-contrast samples.
  • Traditional methods lack orientation selectivity and can introduce artifacts.
  • Vector vortex beams offer potential for advanced optical manipulation.

Purpose of the Study:

  • To develop a multifunctional edge imaging system for orientation-selective enhancement.
  • To enable dynamic polarization control for tunable edge highlighting.
  • To demonstrate the system's utility in microscopy and diagnostics.

Main Methods:

  • Integration of a metasurface-based vector vortex beam generator in a 4f Fourier transform system.
  • Utilizing rotating half-wave plates for tunable spatial polarization control.
  • Experimental validation using resolution charts and yeast cell imaging.

Main Results:

  • Demonstrated real-time visualization of orientation-specific edge features.
  • Achieved selective highlighting of structural details based on orientation.
  • Successful label-free imaging of Saccharomyces cerevisiae.

Conclusions:

  • The developed system provides dynamic, orientation-resolved edge enhancement.
  • This technology is a valuable tool for high-contrast imaging in various scientific and diagnostic fields.
  • Offers improved capabilities over traditional scalar-based edge detection methods.