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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jan 8, 2026

Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

LFC-plus: simultaneous multicolour volume cytometry for high-throughput single-cell analysis.

Zhi Ling^1,2,3,4, Wenhao Liu^1,2, Kyungduck Yoon^1,2,3,4

¹Laboratory for Systems Biophotonics, Georgia Institute of Technology, Atlanta, GA, USA. shu.jia@gatech.edu.

|December 23, 2025

Summary

This summary is machine-generated.

Introducing LFC-plus, a novel light-field cytometry platform for advanced volumetric single-cell analysis. This high-throughput system achieves subcellular resolution, enabling deeper insights in biological research and diagnostics.

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Last Updated: Jan 8, 2026

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Area of Science:

Biotechnology
Cell Biology
Optical Engineering

Background:

Imaging flow cytometry faces challenges balancing resolution, multiplexing, throughput, and complexity.
Existing platforms often struggle to provide simultaneous volumetric and high-resolution single-cell analysis.

Purpose of the Study:

To introduce LFC-plus, a next-generation light-field cytometry platform.
To enable multiparametric, simultaneous multi-color, and volumetric single-cell analysis with enhanced resolution and throughput.

Main Methods:

Integration of model-based image restoration, custom light-field optics, and spectral aperture partitioning.
Development of a platform achieving subcellular resolution in 3D and a large imaging cross-section.
High-throughput data acquisition at nearly 200,000 cells per second.

Main Results:

LFC-plus demonstrates subcellular resolution in all three dimensions.
The platform achieves a near-millimeter-scale imaging cross-section.
Validated across diverse applications: chemotherapy response, cell fusion, and migration analysis.

Conclusions:

LFC-plus is a robust and scalable platform for high-content volumetric cytometry.
The system offers significant advancements for fundamental biology research.
Broad implications for translational diagnostics and high-throughput screening.