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Research progress and applications of reverse genetics systems for infectious bronchitis virus
1Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Animal Disease Prevention and Green Development Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610064, China.
Poultry Science
|December 25, 2025
Summary
Reverse genetics platforms are crucial for studying infectious bronchitis virus (IBV) in poultry. These advanced molecular tools enable precise genome manipulation for developing better vaccines and antiviral strategies against IBV.
Area of Science:
- Veterinary Virology
- Molecular Biology
- Poultry Disease Research
Background:
- Infectious bronchitis virus (IBV) significantly impacts global poultry health.
- Advanced molecular tools are essential for understanding and controlling IBV.
- Reverse genetics offers precise manipulation of the IBV genome.
Purpose of the Study:
- To systematically review reverse genetics platforms for IBV research.
- To examine their development, applications, and challenges.
- To guide researchers in selecting appropriate strategies and inspire innovative solutions.
Main Methods:
- Review of established reverse genetics methods: vaccinia virus systems, in vitro ligation/transcription, RNA recombination, BAC cloning, TAR, and CPR.
- Analysis of principles, advantages, and limitations of each method.
- Discussion of contributions to gene function elucidation and vaccine design.
Main Results:
- Reverse genetics platforms have advanced IBV pathogenesis studies and vaccine development.
- IBV has been developed as a viral vector for multipathogen vaccines.
- Current platforms face technical hurdles and safety considerations.
Conclusions:
- Reverse genetics is a pivotal technology for IBV research and control.
- Future perspectives include integrating CRISPR/Cas9, synthetic biology, and computational approaches.
- Continued innovation in reverse genetics is vital for combating IBV.
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