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PRDM14 promotes the bovine somatic stem cell reprogramming through enhancing oxidative phosphorylation at the initial
Qingqing Wei1, Wenhui Li1, Guina Cui1
1Shandong Provincial Key Laboratory for Livestock Germplasm Innovation & Utilization, College of Animal Science, Shandong Agricultural University, Taian, China.
Gene
|December 25, 2025
Summary
PRDM14 enhances bovine somatic cell reprogramming efficiency by boosting oxidative phosphorylation during early stages. This transcription factor is crucial for pluripotency reacquisition and maintaining bovine induced pluripotent stem cells identity.
Area of Science:
- Stem Cell Biology
- Reproductive Biology
- Molecular Biology
Background:
- Bovine induced pluripotent stem cells (biPSCs) hold promise for regenerative medicine and animal breeding.
- Low reprogramming efficiency and poorly understood mechanisms hinder biPSC applications.
- The role of transcription factor PRDM14 in bovine somatic cell reprogramming is largely unexplored.
Purpose of the Study:
- To investigate the role of PRDM14 in bovine somatic cell reprogramming.
- To elucidate the mechanism by which PRDM14 influences pluripotency.
- To assess the potential of PRDM14 in enhancing biPSC generation and maintenance.
Main Methods:
- Overexpression and knockdown of PRDM14 in bovine embryonic fibroblasts (BEFs).
- Generation of biPSCs using OSKM factors with or without PRDM14.
- Karyotyping, pluripotency marker analysis, differentiation assays, transcriptome analysis, RT-qPCR, and ATP content detection.
Main Results:
- PRDM14 significantly enhances reprogramming efficiency of BEFs by OSKM factors.
- PRDM14 promotes oxidative phosphorylation (OXPHOS) during early reprogramming stages.
- PRDM14 upregulates NANOG and downregulates LIN28, DNMT1/3B, and TET1/2/3 in biPSCs.
Conclusions:
- PRDM14 is a key transcription factor essential for efficient pluripotency reacquisition in bovine somatic cells.
- PRDM14 enhances biPSC generation by modulating metabolic pathways (OXPHOS) and epigenetic regulators.
- PRDM14 plays a critical role in maintaining the identity and pluripotency of bovine induced pluripotent stem cells.
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