Related Experiment Video
Updated: Jan 7, 2026

Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
Coumarin Inhibits Primary Root Growth by Modulating Auxin Signaling via Neddylation
Siqi Liu1,2, Jie Li1,2, Zixuan Zhao1,2
1Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, School of Life Sciences, Jinggangshan University, Ji'an 343009, China.
Abstract:
The allelopathic compound coumarin inhibits root growth across numerous species, but its mechanism is unknown. Through a genetic screen in Arabidopsis mutants, we identified the mutants for AXR1 (AUXIN RESISTANT 1) and ECR1 (E1 C-TERMINAL RELATED 1), two subunits of the NEDD8-activating enzyme (NAE), that are resistant to coumarin. Conversely, overexpression of the NEDD8-encoding gene RUB1 (RELATED TO UBIQUITIN 1) caused hypersensitivity, while the NAE inhibitor MLN4924 blocked coumarin's effect. Since neddylation regulates auxin signaling, we analyzed downstream AUX/IAA proteins and found that the loss-of-function mutant of AUXIN RESISTANT 2 (AXR2) (also known as IAA7) was resistant to coumarin. We further showed that coumarin treatment leads to the accumulation of the AXR2 protein. Taken together, these results demonstrate that coumarin inhibits primary root growth by modulating auxin signaling via neddylation.
More Related Videos
08:04Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes
Published on: October 11, 2024
09:17Investigation of Plant Interactions Across Common Mycorrhizal Networks Using Rotated Cores
Published on: March 26, 2019
Related Concept Videos
Primary and Secondary Growth in Roots and Shoots
Meristems and Plant Growth
Water and Mineral Acquisition
Inhibition of Cdk Activity
Plant Hormones
Cell Signaling in Plants