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Coumarin Inhibits Primary Root Growth by Modulating Auxin Signaling via Neddylation.

Siqi Liu1,2, Jie Li1,2, Zixuan Zhao1,2

  • 1Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, School of Life Sciences, Jinggangshan University, Ji'an 343009, China.

Biology
|December 30, 2025
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Summary

Coumarin inhibits plant root growth by affecting auxin signaling. This study identifies key proteins involved in this process, revealing that coumarin

Keywords:
auxin signalingcoumarinneddylationprimary root growth

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Coumarin is an allelopathic compound known to inhibit root growth in many plant species.
  • The precise molecular mechanism by which coumarin exerts its inhibitory effects on root development remains largely unknown.

Purpose of the Study:

  • To elucidate the mechanism of action of coumarin in inhibiting primary root growth.
  • To identify genetic factors and molecular pathways involved in coumarin's effect on plants.

Main Methods:

  • Genetic screening of Arabidopsis mutants resistant to coumarin.
  • Analysis of mutants in NEDD8-activating enzyme (NAE) subunits (AXR1, ECR1) and RUB1 (a NEDD8 gene).
  • Investigation of auxin signaling pathway components, specifically AUX/IAA proteins like AXR2 (IAA7).

Main Results:

  • Mutants for AXR1 and ECR1, subunits of NAE, exhibited resistance to coumarin.
  • Overexpression of RUB1 led to hypersensitivity, while NAE inhibition blocked coumarin's effect.
  • Loss-of-function mutants of AXR2 (IAA7) were resistant to coumarin, and coumarin treatment caused AXR2 protein accumulation.

Conclusions:

  • Coumarin inhibits primary root growth by modulating auxin signaling through the neddylation pathway.
  • The study implicates the NEDD8-activating enzyme complex and auxin signaling components in coumarin's mechanism of action.
  • Neddylation plays a crucial role in regulating auxin signaling and root growth inhibition by coumarin.