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Updated: Jan 7, 2026
The Clinical Application of Tumor Treating Fields Therapy in Glioblastoma
Published on: April 16, 2019
EFFECT OF ASTAXANTHIN ON THE DEVELOPMENT OF TUMOR- AND RADIATION-INDUCED CHROMOSOMAL DAMAGE IN PERIPHERAL BLOOD
D A Kurinnyi1, O M Demchenko1, O V Zemskova2
1State Institution «National Research Center for Radiation Medicine, Hematology and Oncology of the National Academy of Medical Sciences of Ukraine», 53 Yuriia Illienka St., Kyiv, 04050, Ukraine.
Objective:
to investigate chromosome abnormalities resulting from the tumor induced bystander effect (TIBE) andto assess the ability of astaxanthin to modify TIBE in nonirradiated and in vitro irradiated peripheral blood lymphocytes of glioblastoma patients.
Methods:
Peripheral blood lymphocytes (PBLs) from 30 individuals (20 glioblastoma patients and 10 healthy volunteers)were cultured. Some samples were exposed to γ rays at a dose of 1.0 Gy prior to cultivation. Astaxanthin was added tothe culture medium at a final concentration of 20.0 μg/ml before PBL incubation. Chromosomal preparations wereobtained using standard protocols. Cytogenetic analysis included both chromatid and chromosometype aberrations.
Results:
In the analysis of baseline cytogenetic parameters in nonirradiated peripheral blood lymphocytes ofglioblastoma patients, the mean frequency of aberrant metaphases was (5.91 ± 0.74) %, which was statistically significantly higher than that of the control group (p < 0.05). The mean frequency of chromosome aberrations was6.21 ± 0.87 per 100 cells, indicating the presence of TIBE. Following exposure to ionizing radiation, the level of chromosomal damage was also significantly higher in glioblastoma patients compared with healthy volunteers (p < 0.05).Treatment with astaxanthin in both irradiated and nonirradiated peripheral blood lymphocytes resulted in a significant reduction in the frequency of chromosome aberrations (p < 0.01), primarily due to a decrease in chromosometype aberrations. In irradiated cultures, the frequency of chromosomal aberrations decreased from 23.99 ± 2.24 to12.01 ± 1.21 per 100 cells on average. Notably, astaxanthin did not affect the frequency of single fragments.
Conclusion:
Astaxanthin at a concentration of 20.0 μg/ml significantly reduces the manifestation of the tumorinduced bystander effect at the cytogenetic level in cultures of both nonirradiated and irradiated peripheral bloodlymphocytes from glioblastoma patients.
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