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Metabolomic-based promising technique for fruit-based natural products analysis.

Abdur Rauf1, Ahmed Olatunde2, Zuneera Akram3

  • 1Department of Chemistry, University of Swabi, Swabi, Anbar, 94640, Khyber Pakhtunkhwa, Pakistan.

Food Chemistry: X
|January 1, 2026
PubMed
Summary

Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) reveals diverse bioactive compounds in fruits. This advanced technique enhances understanding of fruit metabolomes for health and industry applications.

Keywords:
BioactivesChemical profilingLiquid chromatography-High resolution mass spectrometryNatural products

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Area of Science:

  • Analytical Chemistry
  • Food Science
  • Pharmacology

Background:

  • Fruit-derived natural products offer significant health and commercial value.
  • Profiling complex chemical compositions is crucial for understanding these products.
  • Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) is a key analytical technique.

Purpose of the Study:

  • To review the application of LC-HRMS for profiling bioactive chemicals in fruits.
  • To highlight the advantages of LC for separation and HRMS for precise mass detection.
  • To discuss the impact of LC-HRMS on fruit metabolomics and natural product discovery.

Main Methods:

  • Utilizing Liquid Chromatography (LC) for separating complex mixtures.
  • Employing High-Resolution Mass Spectrometry (HRMS) for precise mass detection and compound identification.
  • Reviewing existing literature on LC-HRMS applications in fruit analysis.

Main Results:

  • LC-HRMS enables detection of a broad spectrum of bioactive compounds in fruits.
  • The technique provides high precision and resolution, distinguishing isomeric and isobaric compounds.
  • LC-HRMS significantly advances the understanding of fruit metabolomes and their biological roles.

Conclusions:

  • LC-HRMS is the leading method for discovering novel bioactive compounds in fruits.
  • Challenges include complex sample preparation and limited databases, requiring further development.
  • Integration of LC and HRMS drives innovation in health-related and industrial applications of fruit-based natural products.