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Published on: August 8, 2019
Analysis of IFNγ-Induced Invasion of Ovarian Cancer Cells Using 3D Spheroids
Bijaya Gaire1, Ivana Vancurova2
1Department of Oncological Sciences, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. bijaya.gaire@mssm.edu.
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IFNγ is a type II interferon with both anti-tumor and tumor-promoting functions. Since IFNγ is produced in response to cancer chemotherapy and immunotherapy, it is critical to understand its tumor-promoting mechanisms. In ovarian cancer (OC), a short term IFNγ exposure inhibits OC cell invasion, while longer-term exposure induces OC cell invasion; the underlying mechanisms involve IFNγ-induced IL-8 expression. Here, we describe a 3D spheroid culture assay to analyze IFNγ-induced invasion in ovarian cancer cells. The main advantages of this assay include its relevance to tumor biology, reproducibility, and ease of cell manipulations and visualization.
