Bioinformatics Analysis of Bc3, IL-8, and PD-L1 Gene Co-expression in Glioblastoma and Pan-Cancer Using Xena Platform

Identification of key genes regulating critical cellular functions, such as cancer cell proliferation, invasion, and immune escape, is essential for the development of novel cancer therapeutic targets and patient stratification. Our studies have shown that the expression of the proto-oncogene Bcl3 and the pro-angiogenic chemokine IL-8 is increased in ovarian cancer (OC) tissues and correlates with the expression of immune checkpoint PD-L1, resulting in increased proliferation of OC cells. Here, we describe a protocol to analyze the gene co-expression of Bcl3, IL-8, and PD-L1 by using the Xena platform and show that Bcl3, IL-8, and PD-L1 are co-expressed in glioblastoma as well as in pan-cancer samples.