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Related Concept Videos

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Updated: Jan 13, 2026

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Tunable Linkers for Dynamic Thiol-Based Bioconjugation Strategies.

G Cianfoni1, L Pisano1, D M Varouhaki1

  • 1Department of Chemistry and Technology of Drugs, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.

Biomacromolecules
|January 8, 2026
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Summary
This summary is machine-generated.

Recent advances in thiol-based bioconjugation enable controllable and reversible linking of biomolecules for biomedical uses. This review covers disulfide bonds and photocleavable linkers in peptide synthesis, antibody-drug conjugates, and biomaterials.

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Area of Science:

  • Bioconjugation Chemistry
  • Biomedical Applications
  • Materials Science

Background:

  • Thiol-based strategies are crucial in bioconjugation chemistry.
  • These methods offer reversible and stimuli-responsive linkages for biomolecules.
  • Recent innovations focus on advanced thiol-containing linkers.

Purpose of the Study:

  • To critically review the latest developments in thiol-containing linkers.
  • To emphasize their role in controllable and reversible biomolecule conjugation.
  • To explore applications in peptide synthesis, antibody-drug conjugates, and biomaterials.

Main Methods:

  • Literature review covering innovations from the past 15 years (until 2024).
  • Categorization of key classes of cleavable thiol-based linkers.
  • Analysis of their chemical mechanisms and practical implementations.

Main Results:

  • Disulfide bonds and photocleavable groups are key thiol-containing linkers.
  • These linkers enable controllable conjugation for peptide synthesis, antibody-drug conjugates (ADCs), and responsive biomaterials.
  • The review identifies current limitations and future research directions.

Conclusions:

  • Thiol-based bioconjugation offers dynamic and selective platforms.
  • Further research is needed to enhance biocompatibility and functional versatility.
  • Innovations in thiol chemistry are driving progress in biomedical applications.