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Updated: Jul 12, 2026

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
Published on: May 2, 2011
An MVA-Based Vector Expressing the SARS-CoV-2 RBD Fused to N Protein Induces Neutralizing Antibodies and T-Cell
D V Glazkova1, R R Mintaev2, O V Orlova2
1Centre for Strategic Planning and Management of Biomedical Health Risks, Federal Medical-Biological Agency of Russia, Moscow, Russia. dglazkova@cspmz.ru.
Abstract:
Recombinant modified vaccinia virus Ankara (rMVA) viruses expressing two forms of SARS-CoV-2 receptor-binding domain (RBD) of S protein fused to viral N protein were constructed and the immunogenicity of these rMVA was evaluated in BALB/c mice. Vaccination of animals with these vaccine vectors led to the production of SARS-CoV-2 neutralizing antibodies and induced a T-cell response targeting both RBD and N proteins.
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