Potential Biomarkers of Carbohydrate Metabolism Disorders: A Pilot Study

Area of Science:

• Biochemistry
• Analytical Chemistry
• Metabolomics

Background:

• Type 2 diabetes mellitus (T2DM) is a global health concern requiring improved diagnostic methods.
• Metabolic profiling offers a promising avenue for identifying novel biomarkers.
• Current diagnostic approaches may lack sensitivity or specificity for early detection.

Purpose of the Study:

• To perform metabolic profiling of blood, plasma, and erythrocyte mass in patients with T2DM.
• To identify potential biomarkers indicative of T2DM using HPLC.
• To lay the groundwork for further molecular identification and validation.

Main Methods:

• High-performance liquid chromatography (HPLC) was employed for metabolic profiling.
• Analysis focused on plasma and whole blood supernatants.
• Mass spectrometry-based detection was utilized to analyze molecular masses.

Main Results:

• Potential biomarkers for T2DM were identified with specific mass charge numbers: 314.45, 397.45, and 702.55 atomic mass units.
• Distinct spectral patterns were observed in plasma and whole blood samples.
• The identified molecules require further structural elucidation and validation.

Conclusions:

• Metabolic profiling via HPLC can detect potential T2DM biomarkers in blood components.
• The identified mass charge numbers represent promising candidates for T2DM diagnostics.
• Further research is essential to confirm the identity and clinical utility of these potential biomarkers.