3D Cryogel Composites of Modified Purine Derivative as Osteoinductive Agents for Bone Pathologies
Rakhi Bormon1, Ekta Srivastava2, Deepshikha Tewari3
1Department of Chemistry, Centre for Environmental Science and Engineering, Centre for Nanosciences, Mehta Family Centre for Engineering in Medicine, Gangwal School of Medical Sciences and Technology, Indian Institute of Technology Kanpur, Kanpur, Uttar Pradesh, 208016, India.
None:
Purines and their receptors play a role in the regulation of stem cell survival, proliferation, and differentiation. They are responsible for the osteogenic differentiation of stem cells by activating many signaling pathways and enhancing the expression of different osteogenic factors, including bone morphogenetic proteins, runt-related transcription factor 3, alkaline phosphatase (ALP), etc. Here, a 2,6,9-trisubstituted adenine derivative is reported, with negligible cytotoxicity and potent osteogenic potential as demonstrated with mouse myoblast (C2C12 cells) and murine preosteoblasts (MC3T3E1 cells). Osteogenic activity of the derivative (Compound 1) is supported by increased expression of ALP and enhanced calcium deposition observed with alizarin staining within cells. Thus, the reported adenine derivatives can serve as potential therapeutics for bone fractures as an injectable therapeutic opening avenue for cell-free therapy.
More Related Videos
10:19Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs
Published on: August 8, 2022
10:32Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
Published on: May 19, 2023
Related Concept Videos
Bone Remodeling
The Bone Matrix
Bone Disorders
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
Bone Remodeling and Repair
