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Rapid Evaluation of Wet Gluten Content in Wheat Using Hyperspectral Technology Combined with Machine Learning
Yan Lai1, Yan-Yan Li2, Min Sha1,3
1School of Management Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China.
Foods (Basel, Switzerland)
|January 10, 2026
Summary
Machine learning, specifically random forest regression, accurately predicts wheat wet gluten content using hyperspectral data. Optimized visible and near-infrared spectra significantly enhance prediction accuracy for both wheat grains and flour.
Area of Science:
- Agricultural Science
- Spectroscopy
- Machine Learning
Background:
- Accurate and rapid wheat quality evaluation is crucial for the food industry.
- Traditional methods for assessing wheat quality, such as wet gluten content, are often time-consuming and labor-intensive.
- Hyperspectral imaging offers a promising non-destructive technique for analyzing agricultural products.
Purpose of the Study:
- To investigate the performance of various machine learning algorithms for predicting wheat wet gluten content.
- To optimize hyperspectral data preprocessing techniques for improved prediction accuracy.
- To establish a rapid and intelligent method for wheat quality assessment.
Main Methods:
- Collected visible and near-infrared (Vis-NIR) hyperspectral data from wheat grains and flour.
- Applied and compared several machine learning algorithms, focusing on random forest regression (RFR).
- Optimized spectral data using first-derivative (FD) transformation and Savitzky-Golay (SG) filtering.
Main Results:
- Random Forest Regression (RFR) demonstrated superior predictive performance for wet gluten content.
- Optimized visible spectra (SG-filtered FD) achieved high prediction accuracy (r²=0.8579 for grains, r²=0.8383 for flour).
- Fused Vis-NIR data also showed strong predictive power when processed with RFR and optimization techniques.
Conclusions:
- The optimized hyperspectral imaging combined with RFR provides an efficient and accurate method for wheat quality evaluation.
- This intelligent prediction scheme has significant potential for real-time quality control in food processing.
- The study highlights the effectiveness of machine learning in agricultural product analysis.

