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Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
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RGG-motif protein Scd6 affects oxidative stress response by regulating cytosolic caTalase T1 (Ctt1)
Sweta Tiwari1, Chitra Togra1, Sudharshan Sj2
1Department of Biochemistry, Indian Institute of Science, Bangalore, India.
RNA Biology
|January 10, 2026
Summary
Cells use translation repression to manage stress. This study shows Scd6 protein localization during oxidative stress derepresses catalase mRNA, aiding cell survival. This mechanism is conserved in humans.
Area of Science:
- Cellular biology
- Molecular biology
- Stress response mechanisms
Background:
- Cells reprogram gene expression to cope with stress via global translation downregulation and RNA granules.
- Oxidative stress induces genes like catalases, but post-transcriptional regulation is unclear.
- Scd6 protein represses translation by interacting with eIF4G1.
Purpose of the Study:
- To investigate the role of Scd6 in oxidative stress response by regulating cytoplasmic catalase T1 (CTT1).
- To explore the post-transcriptional regulatory events affecting CTT1 during hydrogen peroxide (H2O2) stress.
Main Methods:
- Observing Scd6 puncta formation during peroxide stress using microscopy.
- Assessing cell sensitivity to H2O2 with Scd6 overexpression and deletion.
- Measuring reactive oxygen species (ROS) and Ctt1 protein levels.
- Utilizing single-molecule fluorescence in situ hybridization (smFISH) and RNA immunoprecipitation (RIP).
Main Results:
- Peroxide stress induces Scd6 puncta, separate from P-bodies or stress granules.
- Scd6 overexpression increases H2O2 sensitivity and decreases Ctt1 protein levels.
- Scd6 deletion enhances H2O2 tolerance.
- Stress-induced Scd6 localization to puncta reduces CTT1 mRNA interaction, leading to derepression.
- Human homolog LSm14A shows conserved behavior.
Conclusions:
- Scd6 regulates CTT1 translation during oxidative stress.
- Stress-induced Scd6 relocalization derepresses CTT1 mRNA, enhancing tolerance.
- This stress-induced localization mechanism for translation regulation is conserved across species.
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