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Steady Flow of a Fluid Stream01:27

Steady Flow of a Fluid Stream

Consider a control volume, such as a pipe with solid boundaries, through which fluid flows and changes direction due to the impulse exerted by the resulting force from the pipe walls. In steady flow, the mass of fluid entering the control volume at a given time, t, with velocity v1, is equal to the mass leaving after infinitesimal time dt, with velocity v2.
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Related Experiment Video

Updated: May 11, 2026

Digital Microfluidics for Automated Proteomic Processing
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Temperature-Responsive Agarose-Based Digital Microfluidics: An In-Chip Sample Preparation To Mass Spectrometry

Ling Yan1,2, Jiacheng Fang2, Huimin Zhang3

  • 1Eastern Institute for Advanced Study, Eastern Institute of Technology, Ningbo 315100, China.

Analytical Chemistry
|January 12, 2026
PubMed
Summary

We developed a cost-effective digital microfluidics platform for single-cell proteomics. This accessible workflow streamlines sample processing for mass spectrometry, enabling robust protein identification from trace samples.

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Area of Science:

  • Proteomics
  • Microfluidics
  • Cellular Biology

Background:

  • Mass spectrometry (MS)-based single- and trace-cell proteomics offers valuable cellular insights.
  • Current methods are limited by high cost and complex instrumentation.
  • Need for accessible, scalable workflows for routine multiomics.

Purpose of the Study:

  • To present a cost-effective and accessible workflow for single- and trace-cell proteomics.
  • To demonstrate a scalable solution compatible with standard MS platforms.
  • To overcome limitations of existing microfluidic and cell sorting techniques.

Main Methods:

  • Development of a temperature-responsive agarose-based digital microfluidics (TRA-DMF) platform.
  • One-step sample processing (lysis, reduction/alkylation, digestion) in a parallel four-channel format.
  • Real-time visualization of single-cell capture and high sample recovery (98.3%) with non-pipetting transfer.

Main Results:

  • The TRA-DMF system enables efficient droplet transfer compatible with MS.
  • The complete workflow (TRA-DMF-SCP) takes approximately 3 hours.
  • Over 4000 protein groups identified from 50 cells using a benchtop Orbitrap Fusion MS with high reproducibility.

Conclusions:

  • The TRA-DMF platform provides a practical and scalable solution for trace- and single-cell proteomics.
  • This workflow overcomes MS incompatibility issues of oil-phase microfluidics.
  • Potential for integration into routine multiomics workflows for broader accessibility.