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The Wheat CRK-RLCK-MAPKs Signalling Module Confers High-Temperature All-Stage Resistance to Stripe Rust.

Yifeng Shi1, Yue Xu1, Hai Li1

  • 1State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, Key Laboratory of Plant Protection Resources and Pest Integrated Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, and College of Plant Protection, Northwest A&F University, Yangling, China.

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|January 14, 2026
PubMed
Summary

Scientists identified a new gene, TaCRK6, crucial for wheat's high-temperature all-stage (HTAS) resistance against stripe rust. This discovery aids in breeding climate-resilient wheat by understanding plant defense pathways.

Keywords:
MAPK signalling cascadescysteine‐rich receptor‐like kinaseshigh‐temperature all‐stage resistancereceptor‐like cytoplasmic kinasessalicylic acidstripe rust

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Area of Science:

  • Plant Science
  • Molecular Biology
  • Genetics

Background:

  • Global warming increases wheat vulnerability to stripe rust (Pst).
  • High-temperature all-stage (HTAS) resistance is a novel breeding strategy for climate-resilient wheat.
  • Cysteine-rich receptor-like kinases (CRKs) are key in plant stress response pathways.

Purpose of the Study:

  • Identify genes conferring HTAS resistance in wheat.
  • Elucidate the molecular mechanism of TaCRK6 in stripe rust resistance.
  • Investigate the role of TaCRK6 in signaling pathways.

Main Methods:

  • Gene identification and characterization in wheat cultivar Xiaoyan 6 (XY6).
  • Gene expression analysis under Pst inoculation and high temperature.
  • Gene silencing and overexpression in wheat.
  • Protein-protein interaction and phosphorylation assays.
  • MAPK signaling pathway analysis.

Main Results:

  • TaCRK6 expression is induced by both Pst and high temperatures.
  • Silencing TaCRK6 reduces HTAS resistance, while overexpression enhances it.
  • TaCRK6 interacts with and phosphorylates TaRLCK185, activating the MAPK cascade (TaMAPKKK1-TaMAPKK9-TaMAPK6).
  • The TaCRK6-TaRLCK185-TaMAPKs module regulates salicylic acid (SA) biosynthesis.

Conclusions:

  • A TaCRK6-TaRLCK185-TaMAPKs module mediates dual stress signaling.
  • This module couples with SA pathway initiation to activate HTAS resistance against Pst.
  • TaCRK6 is a vital component for developing climate-resilient wheat cultivars.