Randomised trials in mitral transcatheter edge-to-edge repair: taking yet another look

This protocol presents in detail how to perform real-time echocardiographic guidance during transcatheter mitral valve repair. The fundamental views and the necessary measurements are described for each stage of the procedure.

This protocol demonstrates in detail how to perform fully endoscopic mitral valve surgery (EMS) with percutaneous cannulation of the groin vessels, using a percutaneous plug-based vascular closure device. Fundamental steps and useful instructions are described in detail for each step.

Source: Jose Roberto Moreto, Gustaaf Jacobs and Xiaofeng Liu, Department of Aerospace Engineering, San Diego State University, San Diego, California
The delta wing, shown in Figure 1D, is a popular design in high-speed airplanes due to its superb performance in transonic and supersonic flight regimes. This type of wing has a small aspect ratio and high sweep angle, which reduces drag at high subsonic, transonic and supersonic flight regimes. The aspect ratio is defined as the wing span divided...

In this protocol, we present the experimental procedures of a cell spreading assay that is based on live-cell microscopy. We provide an open-source computational tool for the unbiased segmentation of fluorescently labeled cells and quantitative analysis of lamellipodia dynamics during cell...

The protocol presents a novel stepwise approach to producing a pinna-edge biopsy (PEB) in post-natal day (PND) 7 mice for the purpose of identification and simultaneous...

The full process from brain specimen preparation to serial sectioning imaging using the Knife-Edge Scanning Microscope, to data visualization and analysis is described. This technique is currently used to acquire mouse brain data, but it is applicable to other organs, other...