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Updated: Jan 20, 2026
Irritable Bowel Syndrome I: Introduction
Peoniflorin Alleviates Corticotropin-Releasing Factor-Induced Irritable Bowel Syndrome by Improving the Intestinal
Rijuan Jin1, Zhanyi Zhou2, Yijie Lou3
1Department of Gastroenterology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Key Laboratory of Digestive Pathophysiology of Zhejiang Province, Hangzhou 310053, China.
Abstract:
Using paeoniflorin is a promising strategy for irritable bowel syndrome (IBS) therapy due to its natural origin and pharmacological activity. This study explored its therapeutic effects and mechanisms in corticotropin-releasing factor (CRF)-induced IBS models. In vitro, CRF disrupted tight junction (TJ) in T84 cells via the CRFR1/ROCK2/MLC pathway, downregulating occludin and claudin-1. Paeoniflorin targeted CRFR1, inhibited ROCK2 and MLC phosphorylation, and restored TJ integrity. In vivo, paeoniflorin alleviated anxiety-like behaviors and visceral hypersensitivity in CRF-induced IBS mice, reduced FITC-dextran levels, enhanced goblet cell mucus secretion, and improved intestinal permeability. It also upregulated occludin and claudin-1, restored TJ structure, and modulated the CRFR1/ROCK2/MLC pathway. These findings suggest paeoniflorin as a potential therapeutic agent for IBS-related intestinal barrier dysfunction.
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