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Updated: Jan 20, 2026

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
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Exploring humidity effects on polycrystalline human insulin-ligand complexes: preliminary crystallographic insights

Angelos Kontarinis1, Christina Papaefthymiou1, Stamatina Kafetzi1

  • 1Department of Biology, Section of Genetics, Cell Biology and Development University of Patras Patras GR-26500 Greece.

Journal of Applied Crystallography
|January 19, 2026
PubMed
Summary

Controlling relative humidity (RH) is crucial for maintaining the crystal structure of human insulin (HI) complexes. Organic ligands help stabilize protein crystals, offering insights for drug design and pharmaceutical manufacturing.

Keywords:
SCXRDXRPDhuman insulin complexesin situX-ray powder diffractionligand bindingpharmaceutical crystallographyprotein crystallographyprotein–ligand interactionsrelative humidity effectssingle-crystal X-ray diffractionstructural stabilityunit-cell variation

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