Related Concept Videos

Evaluating Mutation Frequency in Bacteria Using the Beta-Glucosidase Assay04:00

Evaluating Mutation Frequency in Bacteria Using the Beta-Glucosidase Assay

Source: Stefan, A., et al. The Multifaceted Benefits of Protein Co-expression in Escherichia coli. J. Vis. Exp. (96), (2015).This video demonstrates the evaluation of mutation frequency in bacteria using a beta-glucosidase assay. Bacterial cultures expressing a proofreading-deficient DNA polymerase are collected over successive generations. Replication errors caused by reduced proofreading may activate a suppressed beta-glucosidase gene. After centrifugation and permeabilization, a substrate is...
178
In Vitro Measurement of α-Galactosidase A and Acid α-Glucosidase Enzyme Activity03:20

In Vitro Measurement of α-Galactosidase A and Acid α-Glucosidase Enzyme Activity

This video demonstrates the in vitro measurement of enzyme activity for α-galactosidase A and acid α-glucosidase in samples using synthetic 4-Methylumbelliferyl...
604
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Adaptive evolution and isolation techniques are described and demonstrated to yield derivatives of Scheffersomyces stipitis strain NRRL Y-7124 that are able to rapidly consume hexose and pentose mixed sugars in enzyme saccharified undetoxified hydrolyzates and to accumulate over 40 g/L...
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Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield10:18

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Here, we present a protocol to perform ethanol extraction of lignin from several biomass sources. The effect of the extraction conditions on the lignin yield and β-O-4 content are presented. Selective depolymerization is performed on the obtained lignins to obtain high aromatic monomer products.
21.8K
Selective Enrichment and Identification of Salmonella spp. Using Xylose Lysine Deoxycholate and Chromogenic Agar02:10

Selective Enrichment and Identification of Salmonella spp. Using Xylose Lysine Deoxycholate and Chromogenic Agar

Source: Yang, Z., et al. A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.. J. Vis. Exp. (2018)This video demonstrates the selective enrichment and identification of Salmonella using xylose lysine deoxycholate (XLD) and chromogenic agar. Selenite broth promotes Salmonella growth, while XLD agar reveals black-centered colonies through hydrogen sulfide production. Chromogenic agar confirms the identity via enzyme-mediated pigment formation, enabling species-level...
143
The Sol Braiding Method for Handling Thick Hair During Transcranial Magnetic Stimulation: An Address for Potential Bias in Brain Stimulation04:37

The Sol Braiding Method for Handling Thick Hair During Transcranial Magnetic Stimulation: An Address for Potential Bias in Brain Stimulation

Hair type commonly seen in historically underrepresented minorities appears to interfere with transcranial magnetic stimulation (TMS). Here we describe a hair braiding method (The Sol Braiding Technique) that improves...
1.3K