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Composition of Blood Plasma01:24

Composition of Blood Plasma

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Blood plasma is a fluid that contains approximately 92% water and 8% solutes. The solutes include various types of proteins, which constitute about 7% of the total solutes in the plasma. The high-molecular-weight proteins—albumins, globulins, and fibrinogen—are essential to plasma function. Albumins, making up about 60% of the plasma proteins, maintain the osmotic balance within blood vessels by preventing excessive water leakage. Additionally, albumins serve as carrier proteins,...
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Updated: Jan 22, 2026

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
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Microfluidic device for continuous blood plasma separation from whole blood.

Surendran Velmurugan1, Melis Güler Girbas2, Can Dincer3

  • 1Research Center for Microtechnology, FHV - Vorarlberg University of Applied Sciences, Austria; Institute for Synthetic Bioarchitectures, University of Natural Resources and Life Sciences (BOKU), Austria.

Biosensors & Bioelectronics
|January 20, 2026
PubMed
Summary

This study introduces a low-cost microfluidic device for rapid, continuous plasma separation from whole blood. The device significantly reduces red blood cell contamination, offering a promising solution for point-of-care diagnostics.

Keywords:
HemolysisInertial focusingMicrofluidicsPassive hydrodynamicsPoint-of-care diagnosticsWhole blood processing

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Area of Science:

  • Biomedical Engineering
  • Clinical Diagnostics
  • Microfluidics

Background:

  • Plasma separation is crucial for clinical diagnostics but conventional methods are slow and bulky.
  • Current centrifugation techniques are not suitable for point-of-care (POC) applications.

Purpose of the Study:

  • To develop a simple, low-cost microfluidic device for continuous and real-time plasma separation from whole blood.
  • To optimize channel geometry for minimizing red blood cell (RBC) contamination.

Main Methods:

  • Numerical simulations were used to evaluate six channel geometries for optimal RBC separation.
  • Microfluidic devices were fabricated using photolithography and soft lithography.
  • Experimental validation was performed using whole human blood, flow cytometry, and hemolysis assays.

Main Results:

  • The optimized microfluidic device achieved continuous plasma separation with significantly reduced RBC contamination compared to whole blood.
  • Leukocyte content was markedly lower than in plasma from standard centrifugation.
  • Plasma yield reached 3.8% with minimal hemolysis, comparable to centrifuged samples.

Conclusions:

  • The developed microfluidic device offers a robust platform for high-purity plasma separation.
  • This technology has strong potential for enabling point-of-care diagnostic applications.
  • The device provides efficient, real-time plasma separation without external forces.